In the EC-MUG test, if the tube _______, it is positive for E. Coli.

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Multiple Choice

In the EC-MUG test, if the tube _______, it is positive for E. Coli.

Explanation:
The test detects the enzyme activity of β-glucuronidase in E. coli. When the MUG substrate is cleaved by this enzyme, it releases a fluorescent compound that glows under long-wave UV light. So a positive result shows fluorescence under long-wave UV illumination, which is specific to E. coli in this assay. Fluorescence under short-wave UV wouldn’t occur for this substrate, and simply turning yellow or producing gas aren’t indicators used by the EC-MUG test.

The test detects the enzyme activity of β-glucuronidase in E. coli. When the MUG substrate is cleaved by this enzyme, it releases a fluorescent compound that glows under long-wave UV light. So a positive result shows fluorescence under long-wave UV illumination, which is specific to E. coli in this assay. Fluorescence under short-wave UV wouldn’t occur for this substrate, and simply turning yellow or producing gas aren’t indicators used by the EC-MUG test.

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